猪粪中金霉素残留的测定方法.pdfVIP

Acta Agriculturae Zhej iangensis 20(4) : 291~ 295, 2008 1 1 2 1 1 魏瑞成, 王 冉, 李 维 , 陈 明, 郑 勤 1 2 ( , 210014; , 210095) : ( CTC) ( 4 mol/ L = 1316, VVV) C , 0.01 mol/ L 18 = 721414(VVV) , 375 nm CT C 0. 5~ 10.0 mg/ kg , : ( 86.7% 3. 82%) ~ ( 101. 95% 3. 37%) , : 1.45% ~ 7. 11% , R D : 1. 18% ~ 3. 25% , : 2 ng, :0. 5 mg/ kg, , : ; ; : 859. 84 :A : 1004- 1524(2008) 03- 0291- 05 Determination method of chlortetracycline residues in pig faeces 1 1 2 1 1 WEI Ruicheng , WANG Ran , LI Wei , CHENG Ming , ZHENG Qing 1 ( JiangsuKey Laboratory of AnimalDerived Food Saf ety, Jiangsu Academy of Agricultural Science, Nanj ing 10014 , Chi 2 na; College of Animal Sciences, Nanj ing Agricultural University, Nanj ing 10095 , China) Abstract: One method for determining chlortetracycline ( CTC) residues in pig faeces using highperformance liquid chro matography ( HPLCVWD) was developed. The sampleswere extracted with the mixture of acetone, hydrochloride (4 mol/ L) and water in the ratio of 1316(VVV) and centrifuged. Liquid chromatography was performed on C column, the 18 mobile phase was 0. 01 mol/ L oxalic acid, methanol and acetonitrile (721414) , the detection wavelength was at 375 nm.When the added concentration of CTC was between 0. 5- 10.0 mg/ kg, recovery rates were ( 86. 7% 3. 82%) - ( 101. 95% 3. 37%) ,withinday and daytoday R D (relative standard deviation) were 1. 45% - 7. 11% and 1. 18% - 3. 25% respectively. Detection limit was 2 ng and the limit of quantification was 0. 5 mg/ k