熊果酸对ox-LDL诱导人脐静脉内皮细胞氧化损伤的保护作用及其机制与研究.pdfVIP

硕士研究生：苏元镇(心血管内科) 指导教师： 辛辉副教授 关键词：熊果酸；OX—LDL；氧化损伤；人脐静脉内皮细胞；动脉粥样 硬化 The effectofUrsolicacidonhumanumbilicalvein protective endothelialcellsinducedOX—LDLoxidativeand by damage itsmechanism Abstract the of Objective：To Ursolic Acid， investigateprotection Acid(Ursolic humanumbilicalvein UA)on endothelial induced To whethertheeffect damage OX—LDL，and by explore plays l—Nrf2一ARE throughKeap signalingpathways． Methods：HUVECswere for Cultured80％cell and growth density dividedintqintoSIX S： 1I，control， controlwithout gro gro p_joup ： Kroup． ’groups：groupgroup ， without；group HUVECsstimulatedwith endothelialbasalmedium OX-LDL(20mg／L)in for24 Witll treated hours；groupⅢ，HUVECsLDL(20mg／L)and endothelialbasal UA(1．5pmlo／L)in mediumfor24 hours；groupIV， HUVECstreatedwith OX-LDL(20mg／L)andUA(3．Ogrnlo／L)in endothelialbasalmediumfor24 treatedwith V，HUVECs hours；group