- 1、本文档共10页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
《667_ftp》.pdf
Application of Image Analysis in the Fungal Fermentation
of Trichoderma reesei RUT-C30
Viviane Choy, Nilesh Patel, and Jules Thibault
University of Ottawa, ON, Canada K1N 6N5
DOI 10.1002/btpr.667
Published online July 7, 2011 in Wiley Online Library ().
Trichoderma reesei was grown in a stirred-tank bioreactor (STB) and a reciprocating
plate bioreactor (RPB) at four different agitation speeds. A semiautomatic image analysis
protocol that was developed to characterize the mycelium morphology during the fermenta-
tion process based on four morphological types (unbranched, branched, entangled, and
clumped microorganisms) was applied to study the effect of agitation on the morphology of
T. reesei. It was shown via statistical validation that broth samples used for image analysis
represented the whole population of the fungi in the bioreactor. High shear was found to be
damaging to T. reesei grown in the STB. The gentler shear produced in the RPB was not
detrimental to the microorganism even at higher agitation speed. Better productivity was
obtained for T. reesei grown in the STB and the highest productivity, 0.121 IU/mL h, was
obtained at 400 rpm. The morphological parameter, the hyphal growth unit, was found to be
correlated to the productivity. Understanding the effect of agitation on the morphology and
productivity of T. reesei could lead to the design of better bioreactors and the selection of
C
您可能关注的文档
- 《21_ftp》.pdf
- 《21天学通Java6(第5版)》.pdf
- 《21天学通JAVA》.pdf
- 《220V_LED恒流电路》.doc
- 《223TGp_edu_light_v2》.ppt
- 《22_ftp》.pdf
- 《22_How to use QGet to manage multiple BT download jobs remotely》.docx
- 《235_ftp》.pdf
- 《23组基础组合K线图解和十种经典K线组合形态分析》.doc
- 《25 Jazz Fusion Licks Lesson》.pdf
- 分析let s单元56ago2卷纸zheng unit56.pdf
- 塑胶材料其它分类原料pa9t 12.pdf
- md16x16数字媒体切换器设备.pdf
- 者参考项目发起人学科类型单位序列承包商修订页代码顺序典型.pdf
- 届世界天然气大会阿姆斯特丹2006add10288.pdf
- 期测试记录表每周weekly g1g6 journeys tests level 6 lesson26.pdf
- modernize-whitepaper现代化您应用程序白皮书.pdf
- anybackup产品典型案例分析.pdf
- 约克金融工程课程tfeslide32.pdf
- 广州市妇女儿童医疗中心历份教学药历01tjy.pdf
文档评论(0)