重组腺病毒介导E2F-shRNA转移抑制人视网膜母细胞肿瘤生长实验研究.pdf

E2F-shRNA Retinoblastoma RB E2F-shRNA 48 Realtime RT-PCR E2F1 PI ()DNA 50moi Ad-E2F-shRNA Ad-E2F-X X GFP 48 2*105 5d HE E2F-shRNA 48 Realtime RT-PCR 1 hE2F1 sh-RNA 48 PI ( I THE STUDY ON RECOMBINANT ADENOVIRUS MEDIATED E2F-shRNA IN REDUCING THE GROWTH OF RETINOBLASTOMA ABSTRACT Purpose: To investigate the efficacy of Ad-E2F-siRNA in the process of reducing the growth of Retinoblastoma. Method: The certain amount of Retinoblastoma which has been marked with Green Fluorescent Protein (GFP) cultured in the plate were infected by Ad-E2F-siRNA and Ad-E2F-X respectively. The above infected RB cells will be collected after 48h, then counting the number of cells and diluting them in the concentration of 1*105/ml. Injecting the amount of 2*105RB in both eyes , the RB tumor cell injected on the left side was infected with Ad-E2F-siRNA as treatment group, simultaneously the right side is infected with Ad-E2F-X as the control group, all of which will be investigated and taken the consecutive and dynamic photos of the condition of RB tumor’s growth by the special III Fluorescent Microscope within