《yeast one hybrid》.pdfVIP

l a u n a M r e s U Matchmaker™ Gold Yeast One-Hybrid Library Screening System User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Cat. No. 630491 Clontech Laboratories, Inc. PT4087-1 (PR033494) A Takara Bio Company 1290 Terra Bella Ave. Published 30 March 2010 Mountain View, CA 94043 Technical Support (US) E-mail: tech@ Matchmaker™ Gold Yeast One-Hybrid Library Screening System User Manual Table of Contents I. Introduction Protocol Overview 4 II. List of Components7 III. List of Abbreviations 8 IV. Y1HGold Host Strain Information 8 V. Additional Materials Yeast Media Required 9 A. Additional Materials Required for cDNA Amplification, Library Construction, and Screening 9 B. Yeast Media and Supplements Required 9 VI. Constructing the Bait Plasmid and Bait Yeast Strain 10 A. Protocol: Synthesizing and Cloning Your pBait-AbAi Plasmid 10 B. Protocol: Generating the Bait-Reporter Yeast Strains 11 VII. Testing Your Bait Strain for AbAr Expression 13 A. Protocol: Determining the Minimal Inhibitory Concentration of Aureobasidin A for Your Bait 13 VIII. Generating cDNA for Library Construction 14 A. Protocol: First Strand SMART cDNA Sy