《酵母染色体沉淀分析方法Yeast Chromatin Immunoprecipitation (ChIP) Assay》.pdfVIP

中国试剂网 3.25.5 酵母染色体沉淀分析方法YeastChromatinImmunoprecipitation(ChIP)Assay ABSTRACT This protocol describes a method for the detection of proteins bound to specific regionsofchromatininyeast.Therearemanyvariationsofthisassay. MATERIALS Reagents Antibodyofchoice(seeStep21) DOCbuffer TrisCl(10mM,pH8.0) LiCl(0.25M) NP40(0.5%) Deoxycholate(DOC)(0.5%) EDTA(1mM) Dryice Ethanol(100%[icecold],70%) 中国试剂网