《MALDI-TOF-MS》.pdfVIP

1036 J. Fernandez, F. Gharahdaghi and S M. Mische Ekctrophoresis 1998, 19, 1036-1045 Joseph Fernandez Routine identification of proteins from sodium dodecyl Farzin Gharahdaghi Sheenah M. Mische sulfate-polyacrylamide gel electrophoresis (SDS) gels or polyvinyl difluoride membranes using matrix The Rockefeller University RoteidDNA Technology Center, assisted laser desorption/ionization-time of flight-mass New York, NY, USA spectrometry (MALDI-TOF-MS) As the resource laboratory for Rockefeller University our emphasis continues to be on methodology development for the routine analysis of low abundance proteins isolated from native sources. In the past ten years, gel electrophoresis of proteins has become the method of choice for the preparation of micro- gram and submicrogram quantities of protein for primary structural characteri- zation, and over 95% of the samples submitted for protein identification are either in a gel or bound to polyvinyl difluoride membranes (PVDF). As such, we employ multiple microanalytical approaches encompassing Edman sequence degradation, amino acid and matrix assisted laser desorption/ioniza-