异养小球藻的筛选鉴定和其特性研究.pdfVIP

Vol. 1, No. 2 1, 2 M ar. , 2011 Journal of Environm ental Eng ineering Technology 2011 3 : 1674- 991X(2011) 02- 0131- 06 * 李奥搏, 汪苹 , 张正洁, 酒卫敬, 李金穗 , 100048 : 1WLZ- H, 16S rDNA, WLZ- H ( Ch lorella sorok in iana )WLZ- H , KNO WLZ- H ; WLZ- H 3 , ; pHWLZ- H , WLZ- H pHWLZ- H: pH 70, KNO 1 gL, 40 gL, 31 3 : ; ; ; : X 172 : A DOI: 103969.j issn. 1674- 991X. 201102022 Study on Isolation and Identification of a Strain Heterotrophic Ch lorella and its Growth Characteristics LIAo-bo, WANG P in, ZHANG Zheng-jie, JIU W ei-jing, LI Jin-sui College of Chem istry and Environm ental Engineering, Beijing Technology and Business University, B eijing 100048, China Abstract: A heterotroph icW LZ-H strain grow ing fastw as screened from naturalw aters. The cellmorphology of the alga strain w as analyzed w ith scanning electron m icroscope and, in combination w ith 16S rDNA molecu lar b iology identification m ethod, itw as prelmi inarily conclude that theW LZ-H strain belonged toCh lorella sorok in iana. Single environm ental factor test on theW LZ-H strain show ed that the concentration of KNO and glucose had significant 3 effects on W LZ-H strain growth; too h igh temperature would decrease enzyme activity of W LZ-H strain and consequently decrease its growth; pH w ou ld changew ith the growth ofWLZ-H strain and reach the su itable value in different growth phase. The optmi al grow th conditions ofW LZ-H strainw ere: in itial pH 70, KNO content 1