PTDSOD对Nasub2subSsub2subOsub4sub诱导的SHSY5Y细胞损伤的保护和修复作用研究.pdfVIP

PTD-SOD 对Na S O 诱导的SH-SY5Y 细胞损伤的保护和修复作用研究 2 2 4 叶南慧，林艳云，刘树滔，饶平凡*(福州大学生物工程与科学学院，福州 350002) 摘要：目的 研究 PTD-SOD 对 Na S O 诱导的 SH-SY5Y 细胞损伤的保护和修复作用。方法 建立 Na S O 诱导的 2 2 4 2 2 4 SH-SY5Y 细胞缺氧损伤模型，采用MTT 法检测融合蛋白PTD-SOD 对细胞存活率的影响，LDH 释放分析检测PTD-SOD 对细胞损伤后的保护作用及损伤后细胞内氧化与抗氧化水平的变化。结果 对Na S O 诱导的 SH-SY5Y 细胞缺氧损伤， 2 2 4 MTT 活性检测、LDH 分析及氧化与抗氧化水平分析都表明PTD-SOD 能明显提高细胞存活率和细胞内抗氧化能力。结论 PTD-SOD 融合蛋白能保护Na S O 诱导缺氧损伤的 SH-SY5Y 细胞，具有抑制细胞死亡，改善损伤细胞内抗氧化能力。 2 2 4 关键词：融合蛋白；自由基；缺氧损伤；人神经母细胞瘤细胞；抗氧化 中图分类号：R965.2 文献标志码：A 文章编号：1007-7693(2013)01-0131-05 Protective Effects of PTD-SOD Fusion Protein on SH-SY5Y Cell Induced by Sodium Dithionite Sodium Hydrosulfite * YE Nanhui, LIN Yanyun, LIU Shutao, RAO Pingfan (Institute of Biotechnology, Fuzhuo University, Fuzhou 350002, China) ABSTRACT: OBJECTIVE To investigate the protection mechanism of fusion protein PTD-SOD on SH-SY5Y cell against toxicity induced by sodium dithionite sodium hydrosulfite. METHODS Establish the hypoxia damage model of SH-SY5Y cell induced by Na S O . MTT assay was used to measure the survival of SH-SY5Y cell. LDH, SOD and MDA were measured by 2 2 4 spectrophotometric method to analysis the protective effect of PTD-SOD on SH-SY5Y cell and the change of the cell oxidation and antioxidant levels after the hypoxia damage. RESULTS MTT assay demonstrated that PTD-SOD could promote significantly the survival of the cell. LDH release assay and SOD and MDA assay demonstrated that PTD-SOD could promote the levels of antioxidant against the hypoxia