中高浓度异丙酚与瑞芬太尼可影响神经干细胞祖细胞的增殖分化.docVIP

中高浓度异丙酚和瑞芬太尼可影响神经干细胞/祖细胞的增殖分化 细胞内游离钙离子在神经干细胞/祖细胞增殖分化和中枢神经系统发育过程的信号转导中发挥着重要作用 免疫荧光染色显示5.0μg/mL异丙酚使神经干细胞/祖细胞中神经元特异性蛋白β-tubulin表达下降 Article: Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells, by Qing Li1, 2, Jiang Lu1, 2, Xianyu Wang1, 2 (1 Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China; 2 Anesthesiology Research Institude, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China) Li Q, Lu J, Wang XY. Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells. Neural Regen Res. 2014;9(22):2002-2007. 欲获更多资讯： Propofol and remifentanil affect NSC/NPC proliferation and differentiation Intracellular calcium ion concentration ([Ca2+]i) plays an important role as a signal transduction messenger during neural stem/progenitor cell (NSC/NPC) proliferation and differentiation and in the development of the central nervous system. Anesthetics propofol and remifentanil can active γ-aminobutyric acid type A (GABAA) receptor and influence [Ca2+]I in the NSC/NPC. Whether propofol and remifentanil can influence NSC/NPC proliferation and differentiation remains unclear? Qing Li at Taihe Hospital, Hubei University of Medicine, China and his colleagues applied propofol and remifentanil, alone or in combination, at low, moderate or high concentrations (1, 2–2.5 and 4–5 times the clinically effective blood drug concentration), to NSC/NPC from the hippocampi of newborn rat pups. Low concentrations of propofol, remifentanil or both had no noticeable effect on cell proliferation or differentiation; however, moderate and high concentrations of propofol and/or remifentanil markedly suppressed NSC/NPC proliferation and differentiation, and induced a decrease in [Ca2+]i during the initial stage of NSC/NPC differentiation. They therefore proposed that propofol