不同海参多糖化学组成分析比较_盛文静.pdfVIP

Chin J ar Drugs,2007 February, Vol.26 No.1 2007 2 26 1 盛文静,薛长湖, 赵庆喜,徐杰, 李兆杰, 孙妍 ( , 266003) : 选取当前市场销售的品质不一的10 种海参, 析比较其多糖的化学组成 采用酶解法 和乙醇沉淀提取不同海参多糖,以总糖硫酸根糖醛酸氨基糖和中性单糖含量为指标, 进行化学组成 析比 较,并在QSepharose FF 阴离子交换树脂色谱柱上 离, 绘得洗脱曲线 10 种海参多糖的总糖硫酸 根糖醛酸和氨基糖含量的波动范围 别为40.33 %~ 62. 11 %, 19.54 % ~ 29.95 %, 9.85 % ~ 13.38 %和 4.92 %~ 10. 18 % 中性单糖测定表明10 种海参多糖均含有岩藻糖和半乳糖, 部 含有甘露糖和葡萄糖, 且 岩藻糖含量很高10 种海参多糖在阴离子交换树脂色谱柱上的洗脱曲线明显不同, 除仿刺参多糖和日本刺 - 1 参多糖外,其余8 种海参多糖均在盐浓度为3.2 mol L 的洗脱条件下洗脱完全 不同海参多糖在化 学组成上差异明显,其在阴离子交换树脂色谱柱上 离的洗脱曲线也呈现不同特点 :海参; 多糖; 化学组成; 洗脱曲线; 析 : 959.269.06; 931. 74 :A :2007) Chemical component analysis of polysaccharides from different sea cucumbers SHENG Wenjing,XUE Changhu,XU Jie,ZHAO Qingxi,LI Zhaojie,SUN Yan (FoodScience and Technology College of Ocean University of Qingdao, Qingdao 266003, China) Abstract : Objective To analysethe chemical component difference of the polysaccharides sepa rated from ten kinds of sea cucumber, selected from the market. Methods Polysaccharides from different sea cucumbers were separated by papain , precipitated by ethanol, analysed with six chemical indexes, including content of total sugars, sulfate, uronic acid, aminosu gars and neutral monosaccharide, and eluted on QSepharose FF, recording the elution curve. Results Content of total sugars, sulfate, uronic acid and aminosugars of polysaccha rides from ten kinds of sea cucumbers fluctuated within 40. 33 %~ 62. 11 %, 19.54 %~ 29. 95 % , 9.85 %~ 13.38 % and 4. 92 %~ 10. 18 %, respectively. Neutral monosaccharide a nalysis showed that fucose and galactose were in all the ten polysaccharides while mannose and glucose existed partly, and the content of fucose was very high. Elution curve of poly saccharides from ten kinds of sea cucumbers on QSepharose FF differed obviousl