Effect of 17α-ethinylestradiol on the catabolism of high-density lipoprotein apolipoprotein A-I in the rat》.pdfVIP

Atherosclerosis, 67 (1987) 23-31 23 Elsevier Scientific Publishers Ireland, Ltd. ATH 03985 Effect of 17wethinylestradiol on the catabolism of high-density lipoprotein apolipoprotein A-I in the rat Ferdinand M. van ‘t Hooft, Teus van Gent and Arie van To1 Department of Biochemistry I, Medical Faculty, Erasmus University, Rotterdam (The Netherlandr) (Received 26 August, 1985) (Revised, received 27 January, 1986; 3 September, 1986; 11 March, 1987) (Accepted 16 March, 1987) The in vivo metabolism and tissue sites of catabolism of high-density lipoproteins (HDL), labelled specifically in the apolipoprotein (apo) A-I moiety, were studied in rats treated with 17a-ethinylestradiol (EE) for 5 days. Apo A-I was labelled either with 0-(4-do-3-[ *25I]iodobenzoyl)sucrose, a non-degradable labelling compound, or with 1311C1.It was found that EE treatment decreases the serum cholesterol concentration to 10 mg/dl and stimulates the serum decay of apo A-I labelled HDL. The latter effect could be attributed to an increased catabolism of apo A-I labelled HDL in the liver. The increased rates of the serum decay and tissue uptake of apo A-I labelled HDL in EE-treated rats were not affected