Effect of overexpression of human SR-AI on oxLDL uptake and apoptosis in 293T cells》.pdfVIP

International Immunopharmacology 11 (2011) 1752–1757 Contents lists available at ScienceDirect International Immunopharmacology journal homepage: /locate/intimp Effect of overexpression of human SR-AI on oxLDL uptake and apoptosis in , ☆ ☆☆ 293T cells Hong Yang a,⁎, Shi-chao Chen b, Yong-qing Tang c, Ya-lei Dai a,⁎ a Tongji University School of Medicine, Shanghai 200092, China b College of Life Science and Technology, Tongji University, Shanghai 200092, China c Gongli Hospital Pudong New Area, Shanghai, 200135, China a r t i c l e i n f o a b s t r a c t Article history: Type I class A macrophage scavenger receptor (SR)-AI plays an important role in foam cell formation and in Received 3 June 2011 apoptosis in atherosclerosis, however the mechanism remains unclear. Therefore, we generated a pEGFP-C1-SR- Received in revised form 1 July 2011 AI plasmid construct for transient transfection of 293T human embryonic kidney cells and observed if SR-AI Accepted 1 July 2011 expression led: (i) to foam cell formation or apoptosis; and (ii) to expression of apoptosis-related genes Bcl-2 and Available online 21 July 2011 Bak-1 in cells treated with oxidized low-density lipoprotein (oxLDL). The pEGFP-C1 (empty vector) transfected cell line was used as a control. Transfection efficiency of each group was N 90% and transfected cells expressed Keywords: Scavenger receptor