Essential roles of insulin, AMPK signaling and lysyl and prolyl hydroxylases in the biosynthesis and multimerization of adiponectin》.pdfVIP

Molecular and Cellular Endocrinology 399 (2015) 164–177 Contents lists available at ScienceDirect Molecular and Cellular Endocrinology journal homepage: /locate/mce Essential roles of insulin, AMPK signaling and lysyl and prolyl hydroxylases in the biosynthesis and multimerization of adiponectin Lin Zhang a, Ming-Ming Li a, Marie Corcoran a, Shaoping Zhang a, Garth J.S. Cooper a,b,c,d,e,* a School of Biological Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, Faculty of Science, University of Auckland, Auckland, New Zealand b Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand c Department of Pharmacology, Medical Sciences Division, University of Oxford, Oxford OX1 3QT, United Kingdom d Centre for Advanced Discovery and Experimental Therapeutics, Manchester Biomedical Research Centre, Central Manchester University Hospitals NHS Foundation Trust, Manchester M13 9WL, United Kingdom e Centre for Endocrinology Diabetes, Institute of Human Development, Manchester Academic Health Science Centre, The University of Manchester, Manchester M13 9PT, United Kingdom A R T I C L E I N F O A B S T R A C T Article history: Post-translational modifications (PTMs) of the adiponectin molecule are essential for its full bioactivity, Received 9 April 2014 and defects in PTMs leading to its defective production and multimerization have been linked to the mecha- Received in revised form 10 September nisms of insulin resistance, obesity, and type-2 diabetes. Here we observed that, in differentiated 3T3- 2014 L1 a