High-density single nucleotide polymorphisms linkage and quantitative trait locus mapping of the pearl oyster, Pinctada fucata martensii Dunker》.pdfVIP

Aquaculture 434 (2014) 376–384 Contents lists available at ScienceDirect Aquaculture journal homepage: www. elsevi er. com/ locate/ aqua-online High-density single nucleotide polymorphisms linkage and quantitative trait locus mapping of the pearl oyster, Pinctada fucata martensii Dunker Yaohua Shi a, Shi Wang b, Zhifeng Gu a, Jia Lv b, Xin Zhan a, Chengcheng Yu a, Zhenmin Bao b,⁎, Aimin Wang a,⁎ a Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, Haikou 570228, Hainan Province, China b Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, Shandong Province, China a r t i c l e i n f o a b s t r a c t Article history: The pearl oyster, Pinctada fucata martensii, is an important aquaculture species in the production of free marine Received 14 May 2014 pearls in China and Japan; likewise, it is an ideal animal model for the study of biomineralization. A high-density Received in revised form 26 July 2014 genetic linkage map was constructed for P. fucata martensii with 3,117 SNP markers. The map spanned 990.74 cM Accepted 30 August 2014 whereas the genome coverage was 99.1% with an average interval of 0.39 cM, a resolution that was the highest in Available online 6 September 2014 pearl oyster and even higher than that of any other mollusc. QTL mapping and association analysis suggested that