Importance of a suitable working protocol for tape stripping experiments on porcine ear skin Influence of lipophilic formulations and strip adhesion impairment》.pdfVIP

International Journal of Pharmaceutics 491 (2015) 162–169 Contents lists available at ScienceDirect International Journal of Pharmaceutics journa l home page : www.e /locate/ijpharm Importance of a suitable working protocol for tape stripping experiments on porcine ear skin: Influence of lipophilic formulations and strip adhesion impairment a a a a a,b,* Nagelreiter C. , Mahrhauser D. , Wiatschka K. , Skipiol S. , Valenta C. a University of Vienna, Department of Pharmaceutical Technology and Biopharmaceutics, Althanstra ße 14, 1090 Vienna, Austria b University of Vienna, Research Platform ‘Characterisation of Drug Delivery Systems on Skin and Investigation of Involved Mechanisms ’, Vienna, Austria A R T I C L E I N F O A B S T R A C T Article history: The tape stripping method is a very important tool for dermopharmacokinetic experiments in vitro and Received 23 April 2015 the accurate measurement of the removed corneocytes is key for a reliable calculation of a drug’s skin Received in revised form 16 June 2015 penetration behavior. Therefore, various methods to quantify the amount of corneocytes removed with Accepted 18 June 2015 each tape strip have been employed, ranging from gravimetric approaches to protein assays and recently Available online 24 June 2015 near infrared densitometry (NIR) has become very widely used. As this method is based on a reduction of light intensity, interference of formulation components seems conc