Promoter regions of potato vacuolar invertase gene in response to sugars and hormones》.pdf

Plant Physiology and Biochemistry 69 (2013) 9e16 Contents lists available at SciVerse ScienceDirect Plant Physiology and Biochemistry journal homepage: www.else /loc ate/plaphy Research article Promoter regions of potato vacuolar invertase gene in response to sugars and hormones Yongbin Ou, Botao Song, Xun Liu, Conghua Xie, Meng Li, Yuan Lin, Huiling Zhang, Jun Liu* National Centre for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China a r t i c l e i n f o a b s t r a c t Article history: Potato vacuolar acid invertase (StvacINV1) (b-fructofuranosidase; EC 6) has been confirmed to play Received 25 March 2013 an important role in cold-induced sweetening of potato tubers. However, the transcriptional regulation Accepted 22 April 2013 mechanisms of StvacINV1 are largely unknown. In this study, the 50 -flanking sequence of StvacINV1 was Available online 30 April 2013 cloned and the cis-acting elements were predicted. Histochemical assay showed that the StvacINV1 promoter governed b-glucuronidase (GUS) expression in potato leaves, stems, roots and tubers. Quanti- Keywords: tative analysis of GUS expression suggested that the activity of StvacINV1 promoter was suppressed by Cis-acting element sucrose, glucose, fructose, and cold, while enhanced by indole-3-aceti