Proteome and metabolome profiling of wild-type and YCA1-knock-out yeast cells during acetic acid-induced programmed cell death》.pdf

Journal of Proteomics 128 (2015) 173 –188 Contents lists available at ScienceDirect Journal of Proteomics journal h omepage: /locate/jprot Proteome and metabolome profiling of wild-type and YCA1-knock-out yeast cells during acetic acid-induced programmed cell death Valentina Longo a, Maša Ždralević b,1, Nicoletta Guaragnella b, Sergio Giannattasio b, Lello Zolla a,⁎, Anna Maria Timperio a,⁎ a Department of Ecology and Biology, “La Tuscia” University, Viterbo, Italy b Institute of Biomembrane and Bioenergetics, CNR, Bari, Italy a r t i c l e i n f o a b s t r a c t Article history: Caspase proteases are responsible for the regulated disassembly of the cell into apoptotic bodies during mamma- Received 19 May 2015 lian apoptosis. Structural homologues of the caspase family (called metacaspases) are involved in programmed Received in revised form 3 July 2015 cell death in single-cell eukaryotes, yet the molecular mechanisms that contribute to death are currently Accepted 5 August 2015 undefined. Recent evidence revealed that a programmed cell death process is induced by acetic acid (AA-PCD) Available online 9 August 2015 in Saccharomyces cerevisiae both in the presence and absence of metacaspase encoding gene YCA1. Here, we re- port an unexpected role for the yeast metacaspase in protein quality and metabolite control. By using an “omics” Keywords: Yeast approach, we focused our attention on proteins and metabolite