Rapid separation and characterisation of triacylglycerols in ostrich oil by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry》.pdf

Food Chemistry 141 (2013) 2098–2102 Contents lists available at SciVerse ScienceDirect Food Chemistry journal homepage: www.elsevi /locat e/foodchem Analytical Methods Rapid separation and characterisation of triacylglycerols in ostrich oil by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry Yan Zhou a, Ying Xue a, Gen Chun Chen b, An Jiang Zhang c, Zhi Feng Chen b, Xun Liao a,⇑, Li Sheng Ding a,⇑ a Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China b Hangzhou Qianlin Bio-Technology Co., Ltd., Lin’an 311300, China c Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China a r t i c l e i n f o a b s t r a c t Article history: The triacylglycerol (TAG) composition of ostrich oil (Struthio camelus ) was determined by non-aqueous Received 15 November 2011 reversed-phase ultra-performance liquid chromatography–mass spectrometry. Using a column with Received in revised form 8 November 2012 150 mm in length, the TAGs were separated efficiently with acetonitrile/2-propanol gradient at flow rate Accepted 2 May 2013 of 0.2 mL/min for 20 min. Detection is performed by positive APCI mass spectrometry, accurate mass Available online 23 May 2013 measurements (3 ppm) and definite elemental composition for molecular ions and fragment ions were obtained to enable characterisation of 24 TAGs