Reflux induces DNA strand breaks and expression changes of MMP1+9+14 in a human miniorgan culture model》.pdf

E X P E R I M E N T A L C E L L R E S E A R C H 3 1 9 ( 2 0 1 3 ) 2 9 0 5 – 2 9 1 5 Available online at journal homepage: /locate/yexcr Research Article Reflux induces DNA strand breaks and expression changes of MMP1þ9þ14 in a human miniorgan culture model a,n a b c b Annett Sandner , Juliane Illert , Sabine Koitzsch , Susanne Unverzagt , Ilona Schön aDepartment of Otolaryngology, Head and Neck Surgery, Martin-Luther-University Halle-Wittenberg, D-06120 Halle, Germany bDepartment of Otolaryngology, Head and Neck Surgery, Laboratory of Experimental Oncology, Martin-Luther-University Halle-Wittenberg, D-06120 Halle, Germany cInstitute of Epidemiology, Biometry and Informatics, Martin-Luther-University Halle-Wittenberg, D-06097 Halle, Germany a r t i c l e i n f o r m a t i o n a b s t r a c t Article Chronology: Gastroesophageal reflux disease has been implicated in the pathogenesis of adenocarcinoma of Received 23 December 2012 the oesophagus. The same applies to laryngopharyngeal reflux (LPR) and squamous cell cancer of Received in revised form the head and neck, but so far, this link has not been proven. The impact of low pH and bile acids 4 September 2013 has not been studied extensively in cells other than oesophageal cancer cell lines and tissue. Accepted 6 September 2013 The aims of this study were to investigate the pathogenic potential of reflux and its single Available online 25 September 2013 components on the mucosa of the upper re