《降低Pin1对大肠癌SW620细胞的增殖和凋亡能力的影响（英文）》.pdfVIP

Chinese-German Journal of Clinical Oncology February 2011, Vol. 10, No. 2, P100–P104 DOI 10.1007/s10330-011-0737-3 Pin1 expression affects cell proliferation and apoptosis of SW620 cells in colorectal carcinoma* Yan Zhu, Liyuan Qin, Meining Li, Dong Zhang, Yuehong Zhang, Niuliang Cheng Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China Received: 8 November 2010 / Revised: 20 December 2010 / Accepted: 15 January 2011 © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2011 Abstract Objective: The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma. Methods: We constructed a plasmid of RNA interfering (shRNA) for Pin1 gene (pGenesl-1-Pin1), then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation. The protein expression of Pin1 was tested by Western blotting. The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry. In order to explain further the effect of Pin1 in SW620 cells, the protein level of Bcl-2 was analyzed by Western blotting. Results: pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing. The protein relative levels of Pin1 were 0.06 ± 0.04 for the P-shRNA/SW620 cells, and 0.32 ± 0.09 for the P-Con/SW620 cells. The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid, and the apoptotic rate was 12.38% ± 1.55% for the P-shRNA/SW620 group. At the same time, we found that the protein expression of Bcl-2 was also reduced. The results were 0.13 ± 0.04 for the P-shRNA