-蛋白分选与膜泡运输li.pptVIP

A reaction catalyzed by a glycosyltransferase 供体 UDP-N-acetyglucosamine UDP-N-acetyglucosamine 受体 E Quality Control Glucosyltransferase (GT) Quality control （质量控制) of newly synthesized proteinsThe role of N-linked glycosylation（N-连接的糖基化） in ER protein folding Quality control: ensuring that misfolded proteins do not leave ER The lumen of RER contains: GT(glucosyltransferase) recognize unfolded or misfolded proteins and adds a glucose to the end of the oligo saccharide chains. BiP(结合蛋白) and calnexin（钙联接蛋白）or calreticulin(钙网蛋白) (chaperones) : that recognize and bind to unfolded or misfolded proteins and allow them to fold correctly. The Export and Degradation of Misfolded ER Proteins Misfolded soluble proteins in the ER are translocated back into the cytosol, where they are de-glycosylated, ubiquitylated, and degraded in proteasomes. Summary I 8. 1 The endomembrane system（内膜系统） Two categories in eukaryotic cells endoplasmic reticulum（ER）, Golgi complex, endosome (内体)， lysosome（L）, vacuole（V）. the cytosol organelles: mitochondria, chloroplasts, peroxisomes, the nucleus. 8. 2 A few approaches to study the endomembranes A Autoradiography B GFP tagging( Green fluorescence protein) C Subcellular fraction analysis D Cell-free system E Genetic mutants 8.3.1 SER functions A Synthesis of steroid hormones B Detoxification C Release glucose from glucose-6- phosphate. D Sequestering calcium extensively developed in skeletal muscles,kidney tubules, steroid-producing endocrine glands. 8.3.2 function of the rER 1.synthesis of proteins on membrane-bound ribosomes The signal hypothesis 2. processing of newly synthesized proteins 3. synthesis of integral membrane proteins 4. membrane biosynthesis 5. Glycosylation 6. Destruction of misfolded proteins/quality control 思考题： 1。信号肽假说的成分及步骤？ 2。ER中的蛋白质质量监控？ 3. 内膜系统的研究方法？ * * * * * * * * * * * * * * * Electron microscopy reconstruction reveals that a translocon associate