《5-氮胞苷介导的转基因crylAb的阶段复活及其对水稻的生物学效应》.pdfVIP

中国水稻科学(ChineseJRiceSci)，2001．15(1)：63～66 63 Illimar z 吴 刚1崔海瑞1舒庆尧1夏英武1高明尉1 A。T0sAAR Phase of 5-azacytidine—mediatedReactivation andIts Effectsoft crylAbTransgeneBiological the Rice Transgenic Wo Hai—ruil．Srru weil．1llimar 2 Gan91，Cm Qing—ya01·XIAYing—wul．GAOMing ALTOSAAR (Jlnstitutt of ofNwlearAgriculturalScinces，ZhejiangUni—sity．Hangzhou ofOtta删，K1N6Ns．Canada) Bl啦kmi“)，Unitsity ^bnr●ct：Differentconcentrationsof different timewere 5一azacytidine with treatment totreatthe combining employed ricewitha silenced transgenic transcriptionallycrylAbtransgene．Theresultsindicatedthat5-azacylidinecouldreactivate8％ thesilenced rice andthe ofthereactivated as as 一30％of plants expression couldreach transgenic crylAbtransgene high theratalsoluble withIowconcentrationof couldlendtothe reactivation 0．147％of protein．Treatment 5-azacytidine hkbest ratioandthe ofthe with resultin expressioncrylabtransgene．Meanwhile。treatmentazaeytidinecould intiller andthe in showedthat numbers，grainweightperplant，paniclelength study 5-azacytidine delayingflowering．The beusedas in could apotential of andcreationofthe withelite mutagenovercomingtransgenesilencing