《Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by rbcL gene sequencing》.pdfVIP

Acta Oceanologica Sinica 2006, Vol. 25, No. 2, p. 125~ 134 LIU Yongjian et al.! Acta Oceanologica Sinica 2006, Vol. 25, No. 2, p. l25~ 134 http :// e-mail: hyxbe@263.net Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by !#$ gene seguencing 1 1* 1 1 LIU Yongjian , YANG Guanpin , GUAN Xiaojing , MEN Rongxin 1. College of Marine Life Sciences, Ocean University of China, Oingdao 266003, China Received 23 October 2005; accepted 27 January 2006 Abstract Ribulose-1, 5-bisphosphate carboxynase/ oxygenase large subunit gene (rbcl) of Jiaozhou Bay phytoplankton was amplified from spring, summer and autumn surface seawater DNAs and cloned respectively. About 50 clones were randomly selected from each library and seguenced. If identical amino acid seguences are considered as the same operational taxonomy unit (OTU), 61 OTUs are identified according to inferred amino acid seguences, among them, 2 1 from spring seawater, 15 from summer seawater and 25 from autumn seawater. Shannon index calculated from OTU abundances reflects the genetic diversity of a community. The indexes of spring, summer and autumn surface seawater phytoplankton are 2.69, 2.44 and 2.76 respectively, indicating that phytoplankton genetic diversity of autumn seawater is the richest. Seasonal variation of phytoplankton community is significant; the community compositions of three seasons are almost completely different except for the two OTUs shared by summer and autumn. Surface seawater phytoplankton communities are possibly metacommunities di