《Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer》.pdfVIP

Online Submissions: /1007-9327office World J Gastroenterol 2010 April 21; 16(15): 1854-1858 wjg@ ISSN 1007-9327 (print) doi:10.3748/wjg.v16.i15.1854 © 2010 Baishideng. All rights reserved. ORIGINAL ARTICLE Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer Minoru Tomizawa, Fuminobu Shinozaki, Takao Sugiyama, Shigenori Yamamoto, Makoto Sueishi, Takanobu Yoshida Minoru Tomizawa, Department of Gastroenterology, National 3 kinase (PI3K), and PD98059, a specific inhibitor Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, of mitogen-activated protein kinase, were added to Yotsukaido City, Chiba 284-0003, Japan the media. After 72 h, a 3-(4,5-dimethylthiazol-2-yl)- Fuminobu Shinozaki, Department of Radiology, National 5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H- Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, tetrazolium inner salt (MTS) assay was performed to Yotsukaido City, Chiba 284-0003, Japan analyze cell proliferation. A wound assay was performed Takao Sugiyama, Makoto Sueishi, Department of Rheu- to analyze cell motility with hematoxylin and eosin (HE) matology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan staining 48 h after addition of each inhibitor. Shigenori Yamamoto, Department of Pediatrics, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, RESULTS: All cell lines clearl