高密度培养.pptVIP

L/O/G/O High level expression of recombinant BoNT/A-Hc by high cell density cultivation of Escherichia coli 2012.7.22 Recombinant BoNT/A-Hc as a safe and efficacious vaccine for botulism 肉毒神经毒素(BoNT)世界上已知毒性最强的物质之一，共有7种血清型(A～G).其中,A型BoNT毒性最强。其重链(HC)的主要作用是与神经细胞膜上相应受体结合，有抗原决定簇，作为疫苗位点。  摇瓶实验 批次培养 菌株的构建 Click to ad title in here 1 2 3 In this study,the high level expression of the BoNT/A-Hc was achieved by high cell density cultivation of recombinant Escherichia coli in a 2-l batch stirred-tank bioreactor. 补料分批培养 3 4 Bacterial strain and plasmid construction