氨基酸、牛血红蛋白手性识别的电化学地研究.pdfVIP

STUDY ON CHIRAL RECOGNITIONS OF AMINO ACID , HEMOGLOBIN BY ELECTROCHEMISTRY Abstract Chiral molecules are very important kinds of molecules in nature and living things, and widely studied in many areas in sciences. Electrochemical methods generally are simple, fast and suitable for the on-site measurements. In this thesis, chiral electrodes were prepared by chemical modification of chiral molecules, and the recognition and interactions between chiral molecules and biological macromolecules with chiral electrodes were studied by several electrochemical methods such as cyclic voltammetry ，differential pulse, ac electrochemical impedance and open-circuit voltage. The main results obtained are as follows: The carbon paste electrode with silicone oil as the binder was modified with β-cyclodextrin, and used to recognize chirality of tyrosine enantiomer by cyclic voltammetry and differential pulse. The experimental conditions were optimized such as the mixing ratio (0.25%) of graphite powder and β-CD, pH value (7.8), interaction time (70 s). When concentration of chiral tyrosine enantiomers is 0.004mmol/L, only L –tyrosine was recognized and gives out electronic response. The modified electrode can distinguish L –tyrosine from the D-tyrosine. A chiral carbon fiber cluster electrode was prepared firstly by electrochemically deposition of gold thin layer film and then chemically modified by self assemblying of L-cysteine on the surface of gold layer. The recognitions of L-tyrosine with the chiral electrode were studied by ac electrochemical impedance spectrometry. The results show that interaction between L-cysteine modified electrodes and L-tyrosine is stronger than D-tyrosine, and