见血清质量标准分析研究.pdfVIP

目 录 1 见血清质量标准研究„„„„„„„„„„„„„„„„1 1.1 中文摘要„„„„„„„„„„„„„„„„„„„„„1 1.2 英文摘要„„„„„„„„„„„„„„„„„„„„„3 1.3 前言„„„„„„„„„„„„„„„„„„„„„„„5 1.4 仪器和试剂„„„„„„„„„„„„„„„„„„„„7 1.5 方法和结果„„„„„„„„„„„„„„„„„„„„8 1.6 讨论和结论„„„„„„„„„„„„„„„„„„„„25 1.7 参考文献„„„„„„„„„„„„„„„„„„„„„30 1.8 英汉缩略词对照表„„„„„„„„„„„„„„„„„31 2 致谢„„„„„„„„„„„„„„„„„„„„„„„32 3 综述„„„„„„„„„„„„„„„„„„„„„„„33 4 在读期间发表的论文复印件（不含首页、目录） 见血清质量标准研究 摘 要 目的：鉴于目前见血清药材尚无规范的质量标准，仅见收载其来源、性状、 功效，无根、茎、叶、果实、种子及粉末等的显微特征的鉴定标准，无总灰分 及酸不溶性灰分的测定标准，无浸出物和水分等的测定标准。因此，见血清的 质量标准有待于进一步的提高和完善。本研究旨在通过对见血清的质量标准展 开研究，制定其质量标准的草案，对进一步开发和利用见血清资源提供科学依 据。方法：1.对见血清的植物形态和性状进行生药学的鉴定；2.对见血清的根、 茎、叶、果实切片进行显微观察；3.对见血清粉末制片进行显微观察；4.对见 血清的成分进行提取分离，进行初步的理化分析，为进一步确定其有效成分提 供参考；5.按照中国药典2010 版一部附录所载方法测定见血清药材的总灰分、 酸不溶性灰分、水分含量、水溶性浸出物含量；6.用1,3-二亚油酸甘油酯为对 照品对其进行薄层色谱试验，作为见血清质量标准鉴别的方法之一；7.采用酸 碱两相滴定法测定见血清的总生物碱的含量。结果：通过查阅相关文献和进行 合理的实验获得了相应的技术指标和数据：1.对见血清的原植物形态和根、茎、 叶、果实、种子的显微形态进行描述：详见论文相应部分；2.见血清全草水溶 性浸出物热浸法测定含量在20.97%～21.86%，平均含量在21.45% ，故暂定其 水溶性浸出物热浸法不低于 20.0% ；3.见血清含水量在 12.80%～13.20%，平 均值为 12.92%，暂定其水分含量不高于 13.0%；4.见血清总灰分幅动范围在 8.03%～8.78%，平均值为8.43%，故暂定为其总灰分不高于9.0% ；5.见血清 酸不溶性灰分幅动范围在 2.23%～2.57% ，平均值为2.40% ，故暂定见血清酸 不溶性灰分不高于 3.0% ；6.通过酸碱滴定法，测得见血清总生物碱的含量范 围在0.0422%～0.0512%，故暂定见血清总生物碱含量不低于0.04% ；7.薄层 色谱试验发现见血清与1,3-二亚油酸甘油酯具有相同的斑点。结论：本实验通 过对见血清质量标准的研究，完善和提高了见血清的性状鉴别、理化鉴别、显 微鉴别等，其中新增了见血清的粉末鉴别、组织切片观察、总灰分、酸不溶性 灰分、水分、浸出物等的鉴别标准，同时通过酸碱两相滴定法测定了见血清总 生物碱的含量，形成了见血清质量标准的草案，为见血清的进一步开发和利用 提供了科学依据。 关键词：见血清；质量标准；总生物碱; 1,3-二亚油酸甘油酯 The quality standards research of Liparisnervosa Herba Abstract: Objective: Because there is no normative quality standard of Liparis -nervosa Herba, only posted its origin, character, efficacy.No roots, stems, leaves, fruits, seeds and powder characteristics of the evaluation standard, no total ash and acid insoluble ash content inspecttion, no extract and water content of the ex