胼胝体额区有髓神经纤维髓鞘超微结构老年改变的体视学研究.docVIP

胼胝体额区有髓神经纤维髓鞘超微结构老年改变的体视学研究 卢 芳，张 蕾，卢 伟，徐 瑲，姜 蓉，龚 霞，闵琦埕，唐 勇（重庆 400016，重庆医科大学基础医学院组织学与胚胎学教研室，干细胞与组织工程研究室，） [摘要] 目的 探讨大鼠胼胝体额区有髓神经纤维髓鞘超微结构的老年性改变。方法 运用电子显微镜和三维体视学方法分别对年轻组（6月龄）、中老年组（18月龄）和老年组（27月龄）雌性Long-Evans大鼠大脑胼胝体额区有髓神经纤维超微结构进行定量研究。结果 年轻组、中老年组和老年组的有髓神经纤维轴突平均直径（内径）、有髓神经纤维平均直径（外径）、平均内周长、平均外周长、髓鞘平均厚度均未见显著性改变。将有髓神经纤维髓鞘厚度按不同直径分为六个段并分别进行统计分析，结果显示在0.2 - 0.4μm 直径段内，中老年组和老年组髓鞘厚度较年轻组均存在显著降低；在0.4 - 0.6μm直径段内，老年组髓鞘厚度较年轻组存在显著性降低；在0.6 - 0.8μm直径段内，老年组髓鞘厚度较中老年组显著性降低。结论 胼胝体额区有髓神经纤维尤其是小直径的有髓神经纤维髓鞘厚度存在显著老年性降低。 [关键词] 有髓神经纤维；髓鞘；胼胝体额区；体视学 [中图分类号] R329.2+4 [文献标识码] A Stereological study of the age-related changes in the myelin sheath microstructure in the frontal region of corpus callosum Lu Fang, Zhang Lei, Lu Wei, Xu Qiang, Gong Xia, Min Qicheng, Tang Yong（Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, 400016, China ） [Abstract] Objective To explore the age-related changes of the myelin sheath microstructure in the frontal region of corpus callosum. Methods The myelin sheath microstructure in the frontal region of corpus callosum of the young, middle-aged and old-aged female Long-Evans rats were studied by means of electron microscope technique and unbiased stereological techniques. Results There were no significant changes in the mean diameter of axons（inner diameter）， the mean diameter of myelinated fibers（external diameter），the mean inner perimeter，the mean outer perimeter and the mean thickness of the myelin sheathes of the myelinaed nerve fibers in the frontal region of the corpus callosum among young, middle-aged and old aged rats. The thicknesses of the myelin sheaths were divided into six intervals according to the diameter of the myelinated fibers and the mean thickness of the myelinated fibers in each interval was calculated. The mean thickness of the myelin sheaths in the myelinated fibers with the diameter from 0.2 to 0.4μm of middle-aged and old-aged rats was significantly decrease