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Selective Extraction of Bacterial DNA from the Surfaces of Macroalgae.doc

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Selective Extraction of Bacterial DNA from the Surfaces of Macroalgae.doc

Applied and Environmental Microbiology, January 2009, p. 252-256, Vol. 75, No. 10099-2240/09/$08.00+0 ??? doi:10.1128/AEM.01630-08Copyright ? 2009, American Society for Microbiology. All Rights Reserved. Selective Extraction of Bacterial DNA from the Surfaces of Macroalgae Catherine Burke, Staffan Kjelleberg,* and Torsten Thomas School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, The University of New South Wales, Sydney 2052, Australia Received 15 July 2008/ Accepted 27 October 2008 ABSTRACT TopABSTRACTINTRODUCTIONREFERENCES ?A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing. INTRODUCTION TopABSTRACTINTRODUCTIONREFERENCES ?Marine living surfaces are often inhabited by diverse and complex communities of bacteria (2, 13, 14, 17, 18, 35, 36) and hence harbor biodiversity, ecological processes, and biotechnological potential distinct from those of surrounding planktonic communities (7, 19-21, 24, 39, 41). Bacterial epiphytic communities on algae have been studied in terms of nutrient acquisition (5, 10), carbon exchange (4), resistance to biofouling (6, 37), the production of bioactive compounds (1, 14), and, perhaps most notably, the lack of the hosts normal morphological development in the absence of a bacterial community (23, 25, 30, 32). Most studies of marine epibiotic microbial communities have been carried out using cultured isolates; however, it is commonly accepted that the majority of bacteria which exist in the environment are not readily