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Electronic Journal of Biotechnology ISSN: 0717-3458 DOI: 10.2225/vol16-issue6-fulltext-12 RESEARCH ARTICLE Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana 1 1 1 Zhongai Li · Yaqiong Du · Zicheng Wang 1 Henan University, College of Life Science, Plant Germplasm Resources and Genetic Laboratory, Kaifeng, Henan, China Corresponding author: wzc@ Received March 11, 2013 / Accepted August 30, 2013 Published online: November 15, 2013 © 2013 by Pontificia Universidad Católica de Valparaíso, Chile Abstract Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arabidopsis thaliana were successfully conserved in vitro by cryopreservation methods. Results: The effects of osmotic stress due to cryoprotectants during pretreatment and of storage at -196ºC on the stability, the efficiency of genetic transformation and the expression level of exogenous gene were analyzed in Arabidopsis . The results showed that there had not any significant increasing in the efficiency of genetic transformation after cryopreservation, and our observation was not in agreement with earlier reports. Transgenic Arabidopsis lines over-expressing ATOST1 gene were used for the real-time PCR analysis, and the result indicated that the expression of the ATOST1 gene was up-regulated about 2.4-fold in the transgenic seedlings tissues retrieved from cryopreservation than those non-cryopreserved counterparts. Con