白念珠菌野生型CYP51及其H310D、T311A突变型CYP51的克隆、表达及其活性初步测定.pdf

英文缩略词表 Abbreviation 英文缩写 英文全称 Acr acrylamide Amp ampieilline ammonium AP persulfate base bp pair 14Ⅱ一lanosterol CYP5l lanosterolP450 demethylase triphosphate dNTP deoxynucleotide sulfide DMS0 dimethyl dithiothreit01 DTT acid tetraacetic EDTA ethylenediamine flucoazole nu ’ llr 130ur IPTG isopropylthio—B—D-galactoside kilobase Kb liter L medium luria．bertani LB 2．ME beta-mercaptoethanol mln minute minimal concentration MIC inhibitory acid MOPS morpholinepropanesulfonic nonidetP一40 -NP一40 saline PBS phosphate—buffered reaction chain PCR polymerase