《Center for In Vivo Microscopy》.pdf

Magnetic Resonance Histology for Morphologic Phenotyping G. Allan Johnson, PhD Gary P. Cofer, MS, Boma Fubara, BS, Sally L Gewalt, MS, Laurence W. Hedlund, PhD, Robert R. Maronpot, DVM Center for In Vivo Microscopy Bryan Research Building Duke University Medical Center Durham, NC 27710 Address correspondence to: G. Allan Johnson, Ph.D. Telephone: (919) 684-7754 Fax: (919) 684-7158 gaj@ Request reprints from: Sally Zimney Center for In Vivo Microscopy Box 3302 Duke University Medical Center Durham, NC 27710 (919) 684-7758 sallyz@ RUNNING TITLE: MR Histology – Morphologic Phenotyping This work was performed at the Duke Center for In Vivo Microscopy. We gratefully acknowledge support from NIH/NCRR (P41 RR05959) and NIH/NCI R24 CA92656. ABSTRACT Magnetic resonance histology (MRH) images of the whole mouse have been acquired at 100-micron isotropic resolution at 2 .0T with image arrays of 256x256x1024. Higher resolution (50x50x50 microns) of limited volumes has been acquired at 7.1T with image arrays of 512x512x512. Even higher resolution images (20x20x20 microns) of isolated organs have been acquired at 9.4T. The volume resolution represents an increase of 625,000X over conventional clinical MRI. The technological basis is summarized that will allow basic scientists to begin using MRH as a routine method for morphologcic phenotyping of the mouse. MRH promises four unique attributes over conventional histology: a) MRH is non-destructive; b) MRH exploits the unique contrast mechanisms that have made MRI so successful clinically; c) MRH is 3-dimensional; d) the data are inherently digital. We demonstrate the utility in morphologic phenotyping a whole mouse C57BL/6J mouse. Key words: magnetic resonance microscopy, phenotyping, active stain INTRODUCTION The explosive increase in mouse mode