Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh.pdfVIP

Hindawi Publishing Corporation Journal of Parasitology Research Volume 2012, Article ID 467821, 4 pages doi:10.1155/2012/467821 Research Article Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh Mohammad Shafiul Alam,1 Hirotomo Kato,2 Mizuho Fukushige,3 Yukiko Wagatsuma,3 and Makoto Itoh4 1 Parasitology Laboratory, International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka 1212, Bangladesh 2 Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan 3 Department of Epidemiology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Tsukuba 305-8577, Japan 4 Department of Parasitology, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan Correspondence should be addressed to Makoto Itoh, macitoh@aichi-med-u.ac.jp Received 14 March 2012; Accepted 9 April 2012 Academic Editor: Alvin A. Gajadhar Copyright © 2012 Mohammad Shafiul Alam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Mymensingh is the most endemic district for kala-azar in Bangladesh. Phlebotomus argentipes remains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length polymorphism (RFLP) of 18S rRNA gene of sand fly species. Using the RFLP-PCR analysis wi