Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model.pdfVIP

Hindawi Publishing Corporation Advances in Hematology Volume 2012, Article ID 792163, 8 pages doi:10.1155/2012/792163 Research Article Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model Geoffrey R. Holmes,1 Giles Dixon,2 Sean R. Anderson,1 Constantino Carlos Reyes-Aldasoro,3 Philip M. Elks,2, 4 Stephen A. Billings,1 Moira K. B. Whyte,2, 4 Visakan Kadirkamanathan,1 and Stephen A. Renshaw2, 4 1 Department of Automatic Control and Systems Engineering, University of Sheffi eld, Sheffi eld S1 3JD, UK 2 MRC Centre for Developmental and Biomedical Genetics, University of Sheffi eld, Firth Court, Western Bank, Sheffi eld S10 2TN, UK 3 University of Sussex School of Engineering and Design Biomedical Engineering Research Group, Brighton BN1 9QT, UK 4 Department of Infection and Immunity, University of Sheffi eld, Sheffi eld S10 2JF, UK Correspondence should be addressed to Stephen A. Renshaw, s.a.renshaw@sheffield.ac.uk Received 17 February 2012; Accepted 22 April 2012 Academic Editor: Christopher Hall Copyright © 2012 Geoffrey R. Holmes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Neutrophils must be removed from inflammatory sites for inflammation to resolve. Recent work in zebrafish has shown neutrophils can migrate away from inflammatory sites, as well as die in situ. The signals regulating the process of reverse migration are of considerable interest, but remain unknown. We wished to study the behaviour of neutrophils during reverse migration, to see whether they moved away from inflamed sites in a directed fashion in the same way