原创力文档Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse NaCa Exchange in BHK Fibroblasts.PDFVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
A RT I C L E
Massive Ca-induced Membrane Fusion and Phospholipid Changes
Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts
Alp Yaradanakul,1 Tzu-Ming Wang,1 Vincenzo Lariccia,1 Mei-Jung Lin,1 Chengcheng Shen,1
Xinran Liu,2 and Donald W. Hilgemann1
1Department of Physiology and 2Department of Neuroscience, University of Texas Southwestern Medical Center at Dallas,
Dallas, TX 75390
Baby hamster kidney (BHK) i broblasts increase their cell capacitance by 25– 100% within 5 s upon activating maxi-
mal Ca inl ux via constitutively expressed cardiac Na/Ca exchangers (NCX1). Free Ca, measured with l uo-5N,
transiently exceeds 0.2 mM with total Ca inl ux amounting to 5 mmol/liter cell volume. Capacitance responses
are half-maximal when NCX1 promotes a free cytoplasmic Ca of 0.12 mM (Hill coefi cient ≈ 2). Capacitance can
return to baseline in 1–3 min, and responses can be repeated several times. The membrane tracer, FM 4-64, is
taken up during recovery and can be released at a subsequent Ca inl ux episode. Given recent interest in signaling
lipids in membrane fusion, we used green l uorescent protein (GFP) fusions with phosphatidylinositol 4,5-bisphos-
phate (PI(4,5)P2) and diacylglycerol (DAG) binding domains to analyze phospholipid changes in relation to these
responses. PI(4,5)P is rapidly cleaved upon activating Ca inl ux and recovers within 2 min. However, PI(4,5)P de-
y 2 2
g pletion by activation of overexpressed hM
您可能关注的文档
- JAKSTAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation.PDF
- JcTI-I a novel trypsin inhibitor from Jatropha curcas seed cake with potential for bacterial infection treatment.PDF
- Judging whether it is aesthetic Does equilibrium compensate for the lack of symmetry.PDF
- Jump Rope Training Balance and Motor Coordination in Preadolescent Soccer Players.PDF
- Junctional spinal disorders in operated adult spinal deformities present understanding and future perspectives.PDF
- Juvenile-onset inflammatory arthritis a study of adolescents’ beliefs about underlying cause.PDF
- Karyophilic proteins polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.PDF
- Keratin 18 attenuates estrogen receptor α-mediated signaling by sequestering LRP16 in cytoplasm.PDF
- Kidney Patients' Intention to Receive a Deceased Donor Transplant Development of Stage of Change, Decisional Balance, and Self-Efficacy Measures.PDF
- Kinase Activity-dependent Nuclear Export Opposes Stress-induced Nuclear Accumulation and Retention of Hog1 Mitogen-activated Protein Kinase in the Budding Yeast Saccharomyces cerevisiae.PDF
- Massive calcium–activated endocytosis without involvement of classical endocytic proteins.PDF
- Massive endocytosis triggered by surface membrane palmitoylation under mitochondrial control in BHK fibroblasts.PDF
- Matching for Several Sparse Nominal Variables in a Case-Control Study of Readmission Following Surgery.PDF
- Maternal-fetal fluid balance and aquaporins from molecule to physiology.PDF
- Mathematical Biology Modules Based on Modern Molecular Biology and Modern Discrete Mathematics.PDF
- Mathematical Model of Plasmid-Mediated Resistance to Ceftiofur in Commensal Enteric Escherichia coli of Cattle.PDF
- Mathematical model of the Tat-Rev regulation of HIV-1 replication in an activated cell predicts the existence of oscillatory dynamics in the synthesis of viral components.PDF
- Mathematical models of cancer metabolism.PDF
- Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression.PDF
- Maturation of Eukaryotic Ribosomes Acquisition of Functionality.PDF
文档评论（0）