改进检测人类性染色质的方法.docVIP

改进检测人类性染色质的方法 【关键词】 性染色质 　　【Abstract】 AIM: To improve the detection method of human sex chromatin. METHODS: Human buccal mucosal cells were used as materials, the operation of centrifugal dropping slide was improved and the concentration levels were changed in three dyes, Thionin, Giemsa and Aceticgentian violet (AGV). Cover dyed method was employed in staining. RESULTS: Equalized Barr Body Frequency (BBF) was obtained in the samples that had been dyed respectively by Thionin, Giemsa and Aceticgentian violet (20.3±1.4)%, (19.5±1.5)% and (20.0±1.0)% respectively. CONCLUSION: The applicat