己烯雌酚对成年仓鼠睾丸生精的影响.pdfVIP

中国农业科学 2005,38(11):2339-2343 Scientia Agricultura Sinica 己烯雌酚对成年仓鼠睾丸生精的影响 杨筱珍，陈耀星，王子旭 （中国农业大学动物医学院，北京 100094 ） 摘要：探讨环境内分泌干扰物己烯雌酚(DES)对成年动物睾丸生精的影响。将DES皮下注射给成年仓鼠(剂量 为1 mg·kg-1 -1 ·d )，用光镜和电镜观察睾丸和生精细胞形态结构的变化，并用甲基绿-哌咯宁和脱氧核糖核酸末端转 移酶介导的 dUTP 缺口末端标记法（TUNEL）检测生精细胞的凋亡。结果表明，DES 处理后睾丸萎缩，曲精小管变 细。曲精小管内的细胞排列紊乱，大量的精母细胞和精子细胞凋亡，少见长形精子细胞。电镜下，这些退化的精 母和精子细胞的胞浆内有大量髓样或空泡状结构。DES 诱发生精细胞凋亡是造成成年动物睾丸生精障碍的重要原 因。 关键词：DES；成年仓鼠；睾丸；生精细胞；凋亡 Effects of Diethylstilbestrol on Adult Hamster Testes Spermatogenesis YANG Xiao-zhen, CHEN Yao-xing, WANG Zi-xu (College of Animal Medicine, China Agricultural University, Beijing 100094) Abstract: To explore the effects of diethylstilbestrol (DES) an environment endocrine disruptor, on the spermatogenesis of adult hamster, 10 adult hamsters were treated with DES (1mg·kg-1 body weight) daily by subcutaneous injection for one week. Morphological changes on adult hamster testis and spermatogenetic cells were observed by light and transmission electron microscope. Apoptotic spermatogenetic cells were detected by TUNEL staining and methyl green-pyronin staining. The results showed that the testis weight and seminiferous tubule area decreased significantly (P0.01). Germ cells distributed disorderly in seminiferous tubule. Few long spermatids were found in almost all of the tubular lumen. Testis regression was associated with an important increase of apoptosis, especially in spermatocytes and spermatids. A lot of flocculent dense bodies or vacuole structures were observed in