陕、豫两省苹果炭疽病病原鉴定.pdfVIP

中国农业科学 2009,42(9):3224-3229 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.09.026 陕、豫两省苹果炭疽病病原鉴定 张 荣，王素芳，崔静秋，孙广宇 （西北农林科技大学植物保护学院，陕西杨凌 712100 ） 摘要： 【目的】确定引起中国陕、豫两省苹果炭疽病的病原菌种类。【方法】对病原菌进行分离、纯化、致 病性测定以及形态学鉴定、rDNA ITS区序列分析、rDNA ITS区特异引物 CaInt2/ITS4扩增。【结果】自陕西、河 南 11 个地点采集 192 份样本，经鉴定引起陕、豫两省苹果炭疽病的病原菌有 2 种，胶孢炭疽菌[Colletotrichum gloeosporioides (Penz.) Sacc.]和尖孢炭疽菌（C. acutatum J.H. Simmonds）。两种病原菌的区别主要在于分 生孢子形态、菌落颜色、对引物 CaInt2 的特异性等。【结论】首次证实了中国苹果炭疽病可由尖孢炭疽菌（C. acutatum ）引起。两种病原菌在陕、豫两省各地区均有分布，其中，胶孢炭疽菌（C. gloeosporioides ）所占比 例较高。 关键词：尖孢炭疽菌；胶孢炭疽菌；rDNA ITS序列；多样性 Identification of Pathogens Causing Apple Fruit Bitter Rot in Shaanxi and He’nan Provinces ZHANG Rong, WANG Su-fang, CUI Jing-qiu, SUN Guang-yu (College of Plant Protection, Northwest Agricultural and Forestry University, Yangling 712100, Shaanxi) Abstract: 【Objective 】 Determining the possible pathogens of apple fruit bitter rot in Shaanxi and He’nan provinces of China. 【Method 】 The pathogens were isolated and their pathogenicities were determined. Identification was carried out by using traditional morphological methods and sequencing of rDNA-ITS with universal primers and species-specific primer CaInt2. 【Result 】 One hundred and ninety two samples of diseased apple fruits were collected from eleven locations in Shaanxi and He’nan provinces of China. Two fungal species, Colletotrichum gloeosporioides (Penz.) Sacc. and C. acutatum J.H. Simmonds were found as possible pathogens of apple fruit bitter rot in these regions. The two fungal pathogens can be distinguished on the basis of conidial morphology, colony color, growth rate and specific amplification by primers CaInt2/ITS4. 【Conclusio