Preparation and Properties of an ATP-Pi Exchange Complex.pdfVIP

THE JOURNAL 01 BIOI.OGICAL CHEMISTRY Vol. 253, No. 3, Issue of February 10, pp. 956964, 1978 Printed m U S.A. Preparation and Properties of an ATP-Pi Exchange Complex (Complex V) from Bovine Heart Mitochondria* (Received for publication, June 20, 1977) DIANA L. STIGGALL, YVES M. GALANTE,* AND YOUSSEF HATEFI§ From the Department of Biochemistry, Scripps Clinic and Research Foundation, La Jolla, California 92037 Using the general procedure for the isolation of complexes rutamycin, dicyclohexylcarbodiimide, venturicidin, tri- I, II, III, and IV from mitochondria or submitochondrial alkyltin compounds, and mercurials. The ATP-Pi exchange particles, a preparation (complex V) has been isolated from activity is inhibited also by various uncouplers and valino- a fifth fraction with very high ATPJ3Pi exchange (350 to mycin plus nigericin. Complex V catalyzes the hydrolysis 470 nmol . min-’ mg of protein-’ at 30”) and ATPase (8 to of GTP and ITP at appreciable rates, but its exchange D o 10 pm01 . min.’ . mg of protein-’ at 30”) acti