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THE JOURNAL OF BIOLOGICAL CHEMISTRY
Vol. 236, No. 4, April 1961
Printed in U.S.A.
Fractionation of Yeast Amino Acid-Acceptor Ribonucleic
Acids by Countercurrent Distribution*?
B. P. DOCTOR, JEAN APGAR, AND ROBERT W. HOLLEY
From the United States Plant, Soil, and Nutrition Laboratory, Agricultural Research Service,
United States Department of Agriculture and the Department of Biochemistry,
Cornell University, Ithaca, New York
(Received for publication, October 20, 1960)
Considerable interest has developed in recent years in the magnesium chloride, was prepared by dissolving 555 g of dipo-
separation of the amino acid-specific “soluble’‘-fraction ribo- tassium hydrogen phosphate and 870 g of sodium dihydrogen
D
nucleic acids because of the probable role of these RNAs in phosphate monohydrate in sufficient 0.001 M magnesium chloride o
w
protein biosynthesis (l-5) and because the elucidation
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