Purification and Characterization of Human strongTstrong-lymphocyte.pdfVIP

THEJOURNALOF BIOLOGICAL CHEMISTRY Vol. 259, No. 16, Issueof August 25, pp.9992-9996, 1984 0 1984 by The American Society of Biological Chemists, Inc. Printed in U.S.A. Purification and Characterizationof Human T-lymphocyte-derived Erythroid-potentiatingActivity* (Received for publication, December 5, 1983) Carol A. Westbrook$, Judith C. Gassong, Susan E. Gerber, Michael E.Selsted, and David W. Golde From the Division of Hematology-Omobgy, Department of Medicine, UCLA Schoolof Medicine, Los Angeh, California 90024 The human T-lymphoblast cell line, Mo, secretes a row (6). Burst-promoting activity or EPA’ is produced by number of lymphokines, including erythroid-poten- some human monocyte cell lines (7, 8) and most HTLV- tiating activity (EPA), animportant early regulator of infected mature T-lymphoblastlines (9, lo), but not by most erythropoiesis. We report purification of EPA to ho- other human T-cell lines (11).A rich source of EPA is the mogeneity, from serum-free Mo-conditionedmedium. Mo T-cell line which carries the unique HTLV type I1 (12, Purification was accomplished by sequential concen- 13). tration, ammonium sulfate precipitation, lentil lectin We previously reported the partial purification and char- affinity chromatography, gel filtration, and reverse- acterization of Mo T-cell-derived EPA (14). Biologic studies phase high-performance liquid chromatography.EPA suggest