FOXHuntingSystem_一种新功能基因筛选技术.pdfVIP

分子植物育种，2010 年，第8 卷，第4 期，第810-817 页 Molecular Plant Breeding, 2010, Vol.8, No.4, 810-817 技术改进 Upgraded Technology FOX Hunting System———一种新的功能基因筛选技术 1,2 3 1,2* 耿微 高野哲夫 柳参奎 1 东北林业大学, 东北油田盐碱植被恢复与重建教育部重点实验室, 哈尔滨, 150040; 2 东北林业大学盐碱地生物资源环境研究中心, 哈尔滨, 150040; 3 东京大学亚洲生物资源环境研究中心, 东京, 188-0002, 日本 * 通讯作者, shenkuiliu@ 摘 要 FOX hunting system 是近几年发展起来的又一新型分子生物学研究工具。在 2006 年，FOX hunting system 作为一种植物功能获得性基因筛选技术首次被提出，可用于系统性阐明植物基因的功能。 FOX hunting system 可以在转基因植株中过量表达单个或有限数量的全长cDNA ，这些异常表达的全长 cDNA 可能会导致显著的突变表型。根据研究目的对表型等方面发生改变的功能获得性突变植株进行筛 选和解析，可以推断出转基因突变植株中所携带外源基因的功能。本文将就FOX hunting system 的开发、 流程、应用和发展等方面内容进行详细的阐述。 关键词 FOX hunting system, 功能基因, 全长cDNA FOX Hunting System—an Innovatively Functional Genes Screening Tech- nology Geng Wei 1,2 Takano Tetsuo 3 Liu Shenkui 1,2* 1 Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Northeast Forestry University, Harbin, 150040; 2 Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040; 3 Laboratory of Environmental Stress Tolerance Mechanism, Asian Natural Environmental Science Center, University of Tokyo, Tokyo, 188-0002, Japan * Corresponding author, shenkuiliu@ DOI: 10.3969/mpb.008.000810 Abstract In recent years, FOX hunting system is is new developed molecular biological tool. FOX hunting sys- tem was firstly published as an alternative gain-of-function gene hunting technique in 2006, and than it has been developed to identify the genes responsible for mutants of the plants. This system uses overexpression of a single or limited numbers of full length-cDNA (s) in individual transgenic plants. It generates large numbers of dominant mutations, enabling the comprehensive characterization of changed mutants phenotype and identification of the functional genes. In this paper, we give detailed description of technique, such as application, process, application and