捻转血矛线虫基因组学研究进展精要.pptVIP

一 捻转血矛线虫基因组计划 Genome Sequencing Center?Washington University?School of Medicine in St. Louis ?USA The Wellcome Trust Sanger Institut The Wellcome Trust Sanger Institute is a genome research centre set up in 1992 by the Wellcome Trust and the Medical Research Council http://www.sanger.ac.uk 二 捻转血矛线虫基因组 6 chromosomes, approximately 50 Mb in size one of the smallest parasitic nematode genomes less than double the size of some of the larger apicomplexan parasites Toxoplasma gondii has a genome of 35Mb 三 EST数据库 The Wellcome Trust Sanger Institut 10,000 ESTS Genome Sequencing Center 4,843 ESTS (1/2002) 四 基因功能分类 S. Nikolaou, and R.B. Gasser, 2006 Prospects for exploring molecular developmental processes in Haemonchus contortus  Genes or expressed sequence tags (EST) transcribed during development Surface proteins with a structural or environmental-interaction role Proteins identified in excretory/secretory products, with implied roles in immune evasion and/or parasite biology Enzymes related to metabolism or detoxification Intestinal molecules with implied roles in feeding and/or anti-coagulation Signalling molecule 五 基因阶段性表达 Ruurdtje Hoekstra, Allerdien Visser, Myrthe Otsen, Joop Tibben, et al. 2000 EST sequencing of the parasitic nematode Haemonchus contortus suggests a shift in gene expression during transition to the parasitic stages Dadna Hartman , , Debbie R. Donald, Sia Nikolaou, et al. 2001 Analysis of developmentally regulated genes of the parasite Haemonchus contortus  In total, 234 cDNA products 32% were unique to L3 30% were unique to adult Sequence on 70 cloned partial cDNAs 35 each from L3 and adult giving rise to 29 L3 and 26 adult 15 adult and 28 L3 The 15 adult products for further analysis 六 全球群体遗传 Karin Troell, Annie Engstr?m, David A. Morrison, et al. 2006 Global patterns reveal strong population structure in Haemonchus contortus , a nematode parasite of dome