ImmunomodulatoryFunctionofBoneMarrow-DerivedMesenchymalStemCells.ppt

Transcriptome analysis 转录组分析 概念：转录组为功能基因组学在描述基因表达模式的一个概念，指一个细胞内的一套mRNA转录物，包含了某一环境条件、某一生命阶段、某一生理或病理（功能）状态下，生命体的细胞或组织所表达的基因种类和水平。 TRANSCRIPTOME ANALYSIS TRANSCRIPTOME ANALYSIS OF NOD- AND BALB/C-MSC MONITORING MSC TRAFFICKING FOLLOWING INJECTION INTO NOD MICE PREVENTION OF DIABETES BY MSC ISOLATED FROM NOR MICE DISCUSSION 1、Allogeneic MSC are capable of protecting islet mass and of delaying the onset of disease when injected into prediabetic NOD mice. 2、BALB/c-MSC also showed promising results in temporarily reversing hyperglycemia in new-onset diabetic NOD mice. 3、The lack of a long-term effect of BALB/c-MSC may be related to this recognition and subsequent rejection. 4、Additional research is also needed to identify various synergistic immunosuppressive strategies and to optimize prevention and reversal of established diabetes. DISCUSSION 5、Although the use of MSC comprises an exciting therapeutic approach for T1D, translating our results to the clinic requires greater investigation to provide robust preclinical data to safely and effectively use MSC as a cell-based therapy for T1D. DISCUSSION ABSTRACT INTRODUCTION INTRODUCTION INTRODUCTION GROUPING METHODS METHODS MSC morphology and differentiation RESULTS MSC morphology and differentiation MSC flow cytometric characterization 1、Stem cell markers. 2、Costimulatory molecules. 3、Apoptotic factors. MSC flow cytometric characterization CD105 expression was lesser in NOD- than in BALB/c-MSC Neither BALB/c- nor NOD-MSC expressed lymphoid or myeloid markers(CD45 and CD90) MSC flow cytometric characterization Positive costimulatory molecules appeared to be negative in both strains Among negative costimulatory molecules, PD-1 and PD-L2 were not expressed in either strain of MSC (M and O), while PD-L1 was more expressed in BALB/c-MSC compared with NOD-MSC MSC flow cytometric characterization FasL was negative in both NOD- and BALB/c-MSC (Q), but Fas was expressed by both strains To examine MSC in an inflammatory