13_17罗伯逊易位猪POU1F1基因多态性.pdfVIP

HEREDITAS (Beijing) 2008 8 , 30(8): 1015―1020 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.01015 13/17 POU1F1 姜丽花, 赵雯, 何晓波, 张廷荣, 孙金海 , 266109 摘要：采用外周血淋巴细胞培养制备染色体标本, 对 13/17 罗伯逊易位猪的3 种杂交组合的394 头后代进行核 型分析, 出现3 种核型猪：13/17 易位纯合子猪[2n=36, XY 或XX, rob(13;17)] 、13/17 易位杂合子猪[2n=37, XY 或XX, rob(13;17)]和正常核型猪[2n=38, XY 或XX] 。应用PCR-RFLP 技术在POU1F1 基因的 1 746 bp 扩增片段 中检测到1 个Rsa Ⅰ限制性内切酶的多态位点。应用PCR-SSCP 技术检测POU1F1 基因第4 外显子, 在3 种杂 交后代群中均未检测到突变。遗传多态性分析结果表明：Rsa Ⅰ酶切多态位点的突变在3 种杂交后代群中A 等 位基因和AA 基因型频率占优势, 在3 种核型群体中也是A 等位基因和AA 基因型频率占优势, 其中AB 基因型 频率在易位杂合型群体中较高。各杂交后代群均未达到Hardy-Weinberg 平衡, 不同核型群亦处于非平衡状态。 13/17 易位杂合子猪×13/17 易位杂合子猪和13/17 易位杂合子猪×约克两杂交后代群的PIC 表现为中度多态, 而 13/17 易位杂合子猪×皮杜杂交后代群表现为低度多态; 易位杂合型群体表现为中度多态, 正常核型和易位纯合 型群体表现低度多态性。 ：猪; 罗伯逊易位; POU1F1 基因; 核型; 多态性 Polymorphisms of POU1F1 gene in 13/17 Robertsonian translocation pigs JIANG Li-Hua, ZHAO Wen, HE Xiao-Bo, ZHANG Ting-Rong, SUN Jin-Hai College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China Abstract: Karyotype analysis was carried out by means of culturing whole peripheral blood from 394 pigs, which were produced from three hybridization combinations of 13/17 Robertsonian translocation pig. Three types of karyotype were detected in these hybrids, i.e., the homozygous pigs [2n=36, XY or XX, rob (13; 17)], the heterozygous pigs [2n=37, XY or XX, rob (13; 17)], and the normal karyotype pigs [2n=38, XY or XX]. A Rsa Ⅰ restriction enzyme polymorphic site in a 1 746 bp fragment of porcine POU1F1 gene was detected by PCR-RFLP analysis. While no mutation in exon 4 of porcine POU1F1 gene was detected by PCR-SSCP analysis in th