ES小鼠和ES细胞中H19基因甲基化状态.pdfVIP

HEREDITAS (Beijing) 2008 6 , 30(6): 765―770 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00765 ES ES H19 杨荣荣, 周荣艳, 高淑敏, 温晓辉, 李相运 , , 071001 : 为探讨印迹基因H19 的甲基化状态与ES 小鼠胚胎发育之间的关系, 以遗传背景相同的正常成年对照小 鼠、22 只成年ES 小鼠和8 只新生死亡的ES 小鼠以及不同传代次数的ES 细胞为实验材料, 利用甲基化敏感性 限制性内切酶-PCR 技术分别检测了其印迹基因H19 的5′非翻译区两个位点的甲基化状态。结果表明, 发育至 成年的ES 小鼠印迹基因H19 所检测位点的甲基化状态与正常成年对照小鼠之间没有差异, 而新生死亡的ES 小鼠印迹基因H19 所检测位点的甲基化状态与成年ES 小鼠以及正常成年对照小鼠相比则存在明显差异。推 测 ES 细胞中印迹基因 H19 所检测位点的甲基化状态与成年 ES 小鼠以及正常成年对照小鼠之间可能存在 差异。 : ES 细胞; ES 小鼠; DNA 甲基化; 印迹基因H19 Methylation of H19 gene in ES mice and embryonic stem cells YANG Rong-Rong, ZHOU Rong-Yan, GAO Shu-Min, WEN Xiao-Hui, LI Xiang-Yun College of Animal Science and Technology, Agricultural University of Hebei, Cattle Sheep Embryo Engineering Center of Hebei, Bao- ding 071001, China Abstract: To investigate the relationship between the methylation status of imprinted gene H19 and development of mice derived completely from Embryonic stem cells (ES) by tetraploid embryo complementation. The methylation status of two loci at 5 UTR region in imprinted gene H19 in normal adult control mice, 22 adult ES mice, 8 newborn dead ES mice and embryonic stem (ES) cells with different passage number were detected by the using of methylation-sensitive restriction endonuclease-PCR technique. The results indicated that the methylation status of imprinted gene H19 in adult ES mice were identical to that of normal adult control mice. However, some significant differences in the methylation status of imprinted gene H19 were found among newborn dead ES mice, adult ES mice and normal adult control mice. Furthermore, the me- thylation status of imprinted g