Grb10基因在小鼠胚期特异性表达分析.pdfVIP

HEREDITAS (Beijing) 2009 7 , 31(7): 732―740 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2009.00732 Grb10 基因在小鼠胚期特异性表达分析 1 2 1 1 1 1 1 1 刘齐 , 王燕 , 陈岩, 张凤伟, 谷甜甜 , 曲有鹏, 岳磊 , 吴琼 1. , 150001; 2. , 150001 生长因子受体结合蛋白10(Growth factor receptor-bound protein 10, Grb10)是一个存在于小鼠 11 号染色体 和人 7 号染色体的母本表达的印记基因。文章利用原位杂交技术和定量RT-PCR 方法对不同发育阶段的小鼠胚 胎Grb10 基因进行时空表达谱的分析, 以确定该基因在胚胎发育中其表达与组织发育的关系。定量RT-PCR 数 据结果表明, Grb10 在E8.5-E13.5 (Embryonic days 8.5-13.5), 表达水平逐渐增高, 在E13.5 达到高峰, 后期表达 量回落。在胚胎发育的中后期脑、心、肺组织的表达水平呈递减趋势。肝脏组织中Grb10 表达水平较为恒定, 在 E18.5 出现高峰。原位杂交数据验证了定量RT-PCR 的结果, 并且说明了 Grb10 在其他如骨、肾和肌肉等组织 器官的高表达水平。研究结果表明 Grb10 基因是小鼠胚期发育的一个重要的基因。 小鼠; 定量RT-PCR; Grb10; 原位杂交 The expression analysis of Grb10 during mouse embryonic development 1 2 1 1 1 1 LIU Qi , WANG Yan , CHEN Yan , ZHANG Feng-Wei , GU Tian-Tian , QU You-Peng , 1 1 YUE Lei , WU Qiong 1. Developmental Biology Lab of Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China; 2. The Obstetrics and Gynecology Department of the First Hospital Affiliated to Harbin Medical University, Harbin 150001, China Abstract: Grb10 gene expression was analyzed in fetus and four organs during gestation process using the real-time RT-PCR and in situ hybridization. The real-time RT-PCR analysis result showed that the expression of Grb10 in whole em- bryos is throughout E8.5 to E19.5. The Grb10 gene expression level was gradually increased from E8.5 to E13.5, and then gradually reduced. The tissues specific expression levels were decrease