北京油鸡胚胎肝脏来源间充质干细胞的分离培养及其生物学特性.pdfVIP

HEREDITAS (Beijing) 2013 3 , 35(3): 365―372 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2013.00365 北京油鸡胚胎肝脏来源间充质干细胞的分离培养及生 物学特性 1,2 1,2 2 2 1 2 牧仁 , 边艳超 , 浦亚斌 , 李向臣 , 王凤龙 , 关伟军 1. , 010018; 2. , 100193 鸡胎肝间充质干细胞是一种多能干细胞, 可以在体外分化成多种终末分化细胞; 目前大多数肝脏间充质 干细胞研究都集中在大鼠和小鼠, 其他动物研究相对较少。文章从7 日龄北京油鸡胚胎肝脏中分离间充质干细 胞, 原代培养并传代至 15 代。免疫荧光法检测间充质干细胞表面标志物 CD29 、CD44 呈阳性表达, 同时检测 造血祖细胞/肝卵圆细胞的表面标志物 CD34 、CK19 呈阴性表达。RT-PCR 检测 CD29 、CD44 、CD71 、CD73 呈阳性表达。细胞生长曲线呈“S”型。肝脏间充质干细胞通过不同诱导液被成功诱导分化成神经细胞、成骨细 胞。结果表明, 从鸡胎肝中分离获得的间充质干细胞具有和小鼠的间充质干细胞相似的生物学特性及分化潜能, 其具有多向分化的潜能为今后临床广泛应用提供了可能行。 北京油鸡; 间充质干细胞; 增殖能力; 诱导分化 Isolation and biological characterization of mesenchymal stem cells from Beijing fatty chicken fetal liver 1,2 1,2 2 2 1 MU Ren , BIAN Yan-Chao , PU Ya-Bin , LI Xiang-Chen , WANG Feng-Long , GUAN Wei-Jun2 1. College of Animal Veterinary, Inner Mongolia Agricultural University , Hohhot 010018, China; 2. Institute of Beijing Animal Science and Veterinary , Chinese Academy of Agricultural Sciences, Beijing 100193, China Abstract: Mesenchymal stem cells (MSCS) from chicken fetal liver are multipotent stem cells that can differentiate in vitro into various terminally differentiated cells. The majority of studies have focused on rats and mice now. Reports from other animals are less and analyses on domestic animals are few. In this study, chicken liver-derived MSCs were isolated from 7-day-old embryo of Beijing fatty chickens. Primary liver-derived MSCs were subcultured to passage 15. The surface markers of liver-derived MSCs, CD29, and CD44 were detected by immunofluo