消核冲剂对乳腺生病大鼠模型性激素受体及细胞增殖核抗原的影响.pdfVIP

致 谢 三年的研究生学习生活转瞬将逝，回首往事历历在目，其中滋味惟有自知！ 衷心感谢我的导师张福忠教授！导师胸襟开阔、不畏艰难的乐观精神，坚持 自我、大刀阔斧的人格魅力，以及对科学永无止境的追求精神，永远是我人生的 楷模和前进的动力，您的恩情我会铭记心头！ 感谢易维真教授，三年来，您在我的学业上严格要求、不断鞭策，在思想上 给我以谆谆教诲，在生活上无微不至的关心和爱护。您淡薄名利的高尚风格，认 真钻研的学习精神,简朴的生活作风将是我永远学习的榜样！ 感谢中医附院普外科主任于庆生教授,您精深的学术水平，严谨的治学作风， 勇于创新的科学态度使我终生受益，是您一次一次的鼓励让我有了当一名优秀外 科医生的信心，我一定不会辜负您的期望! 感谢师兄沈毅、徐英纳，同学刘书先、刘远成、沈奎在临床实习和课题完成 中所给予的帮助！感谢普外科所有医护人员对我无私的支持和帮助！ 感谢课题研究期间，安徽中医学院第一附属医院病理科窦红漫主任、周导老 师为实验标本的保存及指标的测定所给予的指导和帮助。 最后感谢我最伟大的母亲，是您不变的爱心让我的人生有了新的起点！ 消核冲剂对乳腺增生病大鼠模型性激素受体及细胞增殖核抗原的影响 Abstract Objective:To explore the effect of Xiaohe granules and its influence on estrogen receptor(ER) and progesterone receptor(PR) level , epidermal growth factor receptor (EGFR) level, proliferation cell nuclear antigen （PCNA ）level in galactophore tissues ,phathological changes in estrogen 　induced hyperplasia of mammary glands model of rats.To discuss the drug ’s mechanism for prevertion and treatment of the disease. Methods: 60 unpregnant healthy female Wister rats were randomed into normal group ,model group,Xiaohe group and Tamoxifen(TAM)group,fifteen rats in each group,Except for normal group injected with saline ,all the other three groups were injected with estradiol and progesterone to induce hyperplasia of mammary glands models.The rats were killed and serum was separated from blood after eight weeks .The size of breast were measured,Pathological histological examination was performed on the mammary glands.The expression of ER 、PR 、PCNA 、EGFR in mammary cell were measured by immunohistochemistry. Results: The size of breast of rats by statistics,comparison between model group and Xiaohe group showed significant differences （P